Antibody-antigen complex formation with immobilized immunoglobulins
                    
                        
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منابع مشابه
Antibody-antigen complex formation with immobilized immunoglobulins.
We have investigated the complex formation between an immobilized monoclonal antibody and antigens that differ in size about 50-fold. As a model system, we used an iodinated progesterone derivative and a progesterone-horseradish peroxidase conjugate as tracer and a monoclonal antibody as binding protein. The antibody was immobilized by four different methods: physical adsorption, chemical bindi...
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We glycated immunoglobulins from commercial kits designed to measure human ferritin, thyrotropin, and transferrin, and compared the calibration curves for assays utilizing glycated antibodies with those of assays utilizing non-glycated antibodies. Glycation was verified by borate affinity chromatography and assay with thiobarbituric acid reagent. We found no evidence that antigen-antibody bindi...
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Mouse spleen cells, cultured on surfaces coated with antigen-antibody complexes, are inhibited from responding to the B-cell mitogens, lipopolysaccharide, lipid A, Pneumococcal polysaccharide SIII, and poly I:C. The response to the T-cell mitogen, concanavalin A, is also substantially inhibited by immobilized antigen-antibody complexes, but specific inhibition of the response to phytohemaggluti...
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Specific antibody deficiency (SAD) is a common antibody immunodeficiency defined as a poor antibody response to unconjugated pneumococcal polysccharides present in the 23valent pneumococcal vaccine (PPV23). Clinical manifestations of specific antibody deficiency include recurrent sinopulmonary infections, such as sinusitis, otitis media, bronchitis, and pneumonia. All immunoglobulin concentrati...
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Immune sera were subjected to treatment with alkali in the cold to cause dissociation of antigen-antibody complexes. Precipitates and a capacity to fix complement developed in some of these sera subsequent to such treatment. The specific immunologic nature of these phenomena and their observation in relation to disappearance of free antigen and appearance of free antibody in circulation are dis...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1992
ISSN: 0003-2697
DOI: 10.1016/0003-2697(92)90577-t